Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Photosynthesis by intact isolated chloroplasts on solid support.

Z G Cerovic1, J K Cheesbrough, D A Walker

  • 1Institute of Botany and Botanical Garden, Faculty of Science, University of Belgrade, 11000 Belgrade, Yugoslavia.

Plant Physiology
|August 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hierarchical subdivision of Arctic tundra based on vegetation response to climate, parent material and topography.

Global change biology·2022
Same author

THE EFFECT OF THE COLLOIDAL STATE OF THE EMULSION ON ICE CREAM STRUCTURE.

Journal of texture studies·2017
Same author

Data on the number and frequency of scientific literature citations for established medulloblastoma cell lines.

Data in brief·2016
Same author

Reply to Comment on: The UK Experience of a Treatment Strategy for Pediatric Metastatic Medulloblastoma Comprising Intensive Induction Chemotherapy, Hyperfractionated Accelerated Radiotherapy, and Response-Directed High-Dose Myeloablative Chemotherapy or Maintenance Chemotherapy (Milan Strategy).

Pediatric blood & cancer·2016
Same author

Learning from Semmelweis: engaging in sensible infection control.

Anaesthesia·2014
Same author

Rich and cold: diversity, distribution and drivers of fungal communities in patterned-ground ecosystems of the North American Arctic.

Molecular ecology·2014
Same journal

Autoinhibition of Plasma Membrane H+-ATPase1 Regulates Systemic Herbivore Defense in Arabidopsis.

Plant physiology·2026
Same journal

A deep learning model captures position-specific preferences of plant regulatory sequences and suggests genes under complex regulation.

Plant physiology·2026
Same journal

Nissolia brasiliensis as a non-nodulating model legume.

Plant physiology·2026
Same journal

Auxin response factor OsARF22 controls rice seed vigor by suppressing ABA signaling.

Plant physiology·2026
Same journal

The primary nitrate response TGA1 and TGA4 transcription factors are negative regulators of sulfate uptake and metabolism.

Plant physiology·2026
Same journal

TaSPL14-D diverged from its ortholog to regulate tiller angle in rice: a caveat for orthology-based functional inference.

Plant physiology·2026
See all related articles

Researchers developed a new method to measure photosynthesis in isolated chloroplasts. This technique allows for more accurate in vitro assessments of photosynthetic gas exchange, mimicking conditions within plant leaves.

Area of Science:

  • Plant Physiology
  • Biochemistry
  • Cell Biology

Background:

  • Accurate measurement of photosynthesis is crucial for understanding plant productivity.
  • Previous methods for studying isolated chloroplasts had limitations in replicating in vivo conditions.

Purpose of the Study:

  • To develop a novel and improved approach for measuring photosynthesis in isolated chloroplasts.
  • To enable in vitro photosynthesis measurements that more closely resemble the environment within plant leaves.

Main Methods:

  • Isolated chloroplasts were immobilized within the cavities of membrane filters.
  • Thin layers of chloroplasts were subjected to gas exchange measurements (O(2) evolution and CO(2) assimilation) in specialized leaf-chambers.
  • Both closed and flow-through systems were utilized for gas exchange analysis.

Related Experiment Videos

  • Chloroplast integrity was confirmed using light and scanning electron microscopy.

    • Main Results:

    • The developed method successfully demonstrated photosynthetic gas exchange (O(2) evolution and CO(2) assimilation) in isolated chloroplasts.
    • Chloroplasts maintained morphological integrity and stable photosynthetic rates when supplied with phosphate and alkaline phosphatase.
    • The system allowed for measurements under both closed and flow-through conditions.

    Conclusions:

    • The membrane filter trapping technique provides a robust method for in vitro photosynthesis measurements.
    • This approach enhances the physiological relevance of isolated chloroplast studies by mimicking leaf conditions.
    • The method facilitates detailed investigation into chloroplast function and photosynthetic processes.