Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzyme activities associated with maize kernel amyloplasts.

E Echeverria1, C D Boyer, P A Thomas

  • 1Department of Horticulture, The Pennsylvania State University, University Park, Pennsylvania 16802.

Plant Physiology
|March 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Surgical emphysema reduction: A viable alternative in the era of endobronchial valves].

Revue des maladies respiratoires·2025
Same author

Controlled donation after circulatory death lung transplantation: Results of the French protocol including in situ abdominal normothermic regional perfusion and ex vivo lung perfusion.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation·2023
Same author

Comparing critical speed modelling approaches and exploring relationships with match-play variables in elite male youth soccer players.

South African journal of sports medicine·2023
Same author

The evolutionary heritage and ecological uniqueness of Scots pine in the Caucasus ecoregion is at risk of climate changes.

Scientific reports·2021
Same author

Scholarship in Teaching: An Approach to Enhancing the Value and Academic Standing of Teaching.

Medical science educator·2021
Same author

[Treatment efficacy of dietary supplement Licofor for dry eye associated with meibomian gland dysfunction].

Zhonghua yi xue za zhi·2021
Same journal

Nissolia brasiliensis as a non-nodulating model legume.

Plant physiology·2026
Same journal

Auxin response factor OsARF22 controls rice seed vigor by suppressing ABA signaling.

Plant physiology·2026
Same journal

The primary nitrate response TGA1 and TGA4 transcription factors are negative regulators of sulfate uptake and metabolism.

Plant physiology·2026
Same journal

TaSPL14-D diverged from its ortholog to regulate tiller angle in rice: a caveat for orthology-based functional inference.

Plant physiology·2026
Same journal

From wrinkled seeds to plant oil accumulation networks: The legacy of a Plant Physiology classic.

Plant physiology·2026
Same journal

LcHXK1 mediates glucose signaling to inhibit fruit abscission by phosphorylating LcWRKY42, a feedback regulator in lignin polymerization.

Plant physiology·2026
See all related articles

Maize amyloplasts contain enzymes for starch synthesis, preferring triose phosphate as a substrate. Sucrose must be broken down in the cytosol before entering amyloplasts for starch production.

Area of Science:

  • Plant biochemistry
  • Starch metabolism in maize endosperm

Background:

  • Amyloplasts are key organelles for starch synthesis in plants.
  • Understanding enzyme localization and substrate preference is crucial for maize kernel development.

Purpose of the Study:

  • To investigate the enzymatic activities within maize endosperm amyloplasts related to gluconeogenesis and starch metabolism.
  • To determine substrate uptake and incorporation into starch by isolated amyloplasts.

Main Methods:

  • Isolation of amyloplasts from 14-day-old maize endosperm.
  • Enzyme activity assays for gluconeogenesis and starch metabolism enzymes.
  • Substrate uptake and starch incorporation experiments using radioactively labeled compounds.

Main Results:

Related Experiment Videos

  • Key enzymes for starch synthesis (e.g., ADPG pyrophosphorylase, starch synthases) were detected in amyloplasts.
  • Only phosphorylated substrates (like dihydroxyacetone phosphate) were incorporated into starch, with uridine triphosphate enhancing the process.
  • Cytoplasmic enzymes like alcohol dehydrogenase and invertase were largely absent from purified amyloplasts.

Conclusions:

  • Amyloplasts possess the necessary enzymes to convert triose phosphates into starch.
  • Sucrose is metabolized in the cytosol before entering amyloplasts.
  • Uridine triphosphate may be the preferred nucleotide for starch synthesis in developing maize kernels.