Nuclear-Cytoplasmic Partitioning of Phytochrome-Regulated Transcripts in Pisum sativum
A D Sagar1, W R Briggs, W F Thompson
1Carnegie Institution of Washington Department of Plant Biology, Stanford, California 94305.
Abstract:
Nuclear and cytoplasmic mRNAs for several phytochrome-regulated genes were examined in Pisum seedlings in order to investigate possible light effects on mRNA partitioning between the nucleus and cytoplasm. Transcripts from each of five light-regulated genes exhibited different responses to a variety of light treatments, but for each transcript we observed a characteristic linear relationship between nuclear and cytoplasmic levels over a wide range of total transcript abundance. Different mRNAs are characterized by different nuclear-cytoplasmic ;partitioning coefficients', indicating that post-transcriptional events play a significant role in regulating the accumulation of these mRNAs during light induction.
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