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Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor
Published on: January 12, 2019
Monoclonal antibodies to 13-deoxy-gibberellins
J P Knox1, M H Beale, G W Butcher
1Monoclonal Antibody Centre, AFRC Institute of Animal Physiology and Genetics Research, Cambridge Research Station Babraham, Cambridge CB2 4AT, U.K.
Abstract:
The production and characterization of two high affinity rat monoclonal antibodies to 13-deoxy-gibberellins is described. Hybrid myelomas were derived from rats immunized with an immunogenic keyhole limpet hemocyanin-gibberellin conjugate, linked at carbon-3 to gibberellin A(4) via a hemisuccinate bridge. The selected monoclonal antibodies were characterized by a competitive radioimmunoassay.
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