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Related Experiment Videos

Low CO(2) Prevents Nitrate Reduction in Leaves.

W M Kaiser1, J Förster

  • 1Lehrstuhl Botanik I der Universität, Mittlerer Dallenbergweg 64, D-8700 Würzburg, Federal Republic of Germany.

Plant Physiology
|November 1, 1989
PubMed
Summary

Carbon dioxide assimilation is crucial for nitrate reduction in spinach leaves. Water stress and CO(2) deficiency inhibit nitrate reductase activity, which is rapidly reversible upon CO(2) reintroduction.

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Area of Science:

  • Plant Physiology
  • Biochemistry

Background:

  • Nitrate reduction is an essential metabolic process in plants.
  • The relationship between photosynthesis and nitrate assimilation is complex.
  • Nitrate reductase (NR) is a key enzyme in nitrate assimilation.

Purpose of the Study:

  • To investigate the correlation between carbon dioxide (CO(2)) assimilation and nitrate reduction in spinach leaves.
  • To determine the effects of water stress and CO(2) deficiency on nitrate reductase activity.
  • To elucidate the regulatory mechanisms linking nitrate reductase to photosynthesis.

Main Methods:

  • Measuring light-dependent changes in leaf nitrate levels under varying water and CO(2) conditions.
  • Assessing extractable nitrate reductase (NR) activity.
  • Monitoring malate accumulation as an indicator of nitrate reduction.

Main Results:

  • Detached spinach leaves reduced stored and supplied nitrate in the light.
  • Mild water stress inhibited nitrate reduction, but this was reversed by elevated CO(2) levels.
  • CO(2) deficiency or absence inhibited nitrate reduction and rapidly decreased NR activity.
  • NR activity showed rapid inactivation/activation, correlating with CO(2) availability.

Conclusions:

  • Nitrate reduction in spinach leaves is tightly coupled to CO(2) assimilation and net photosynthesis.
  • A rapid post-translational regulation mechanism for NR activity exists, dependent on CO(2) levels.
  • Water stress indirectly affects nitrate reduction by limiting CO(2) availability through stomatal closure.

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