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Updated: Aug 8, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
Phosphoinositides in barley aleurone layers and gibberellic Acid-induced changes in metabolism
P P Murthy1, J M Renders, L M Keranen
1Department of Chemistry and Chemical Engineering, Michigan Technological University, Houghton, Michigan 49931.
Abstract:
Phospholipids of barley (Hordeum vulgare L. cv Himalaya) aleurone layers were labeled with myo-[2-(3)H]inositol or [(32)Pi], extracted, and analyzed by physical (chromatography) and chemical (deacylation) techniques. Three phospholipids were found to incorporate both myo-[2-(3)H]inositol and [(32)Pi]-phosphatidylinositol, phosphatidylinositol-monophosphate, and phosphatidylinositol-bisphosphate. Stimulation of [(3)H]inositol prelabeled aleurone layers with GA(3) showed enhanced incorporation of label into phosphatidylinositol within 30 seconds and subsequent rapid breakdown. Stimulation of phosphatidylinositol labeling observed in these studies is the earliest response of aleurone cells to gibberellic acid reported.
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