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Related Experiment Videos

Polygalacturonase Isozymes and Pectin Depolymerization in Transgenic rin Tomato Fruit.

D Dellapenna1, C C Lashbrook, K Toenjes

  • 1Mann Laboratory, Department of Vegetable Crops, University of California, Davis, California 95616.

Plant Physiology
|December 1, 1990
PubMed
Summary

Tomato fruit ripening involves polygalacturonase (PG) isozymes. This study shows that the PG1 isozyme alone is sufficient for polyuronide solubilization and depolymerization in transgenic rin tomatoes.

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Area of Science:

  • Plant Molecular Biology
  • Fruit Ripening Biochemistry
  • Enzymology

Background:

  • Tomato (Lycopersicon esculentum) fruit ripening is a complex process involving cell wall modifications.
  • Polygalacturonase (PG) is a key enzyme in fruit softening, with multiple isozymes (PG1, PG2A, PG2B) identified.
  • Mutant (rin) tomatoes exhibit impaired ripening, providing a model to study specific ripening-associated gene functions.

Purpose of the Study:

  • To investigate the origin and function of different polygalacturonase (PG) isozymes during tomato fruit ripening.
  • To determine if all three PG isozymes (PG1, PG2A, PG2B) are necessary for polyuronide solubilization and depolymerization in vivo.

Main Methods:

  • Construction and expression of a chimeric gene for developmentally regulated tomato polygalacturonase in ripening-impaired (rin) mutant tomato fruit.

Related Experiment Videos

  • Analysis of polygalacturonase isozyme accumulation in transgenic rin fruit.
  • Measurement of polyuronide solubilization and depolymerization activities in transgenic rin fruit.

    • Main Results:

    • Expression of the chimeric PG gene in rin fruit led to the accumulation of all three PG isozymes (PG1, PG2A, PG2B).
    • Maximal polyuronide solubilization and depolymerization occurred in transgenic rin fruit before the appearance of PG2 isozymes.
    • These findings indicate that PG1, PG2A, and PG2B result from the differential processing of a single gene product.

    Conclusions:

    • The three major polygalacturonase isozymes (PG1, PG2A, PG2B) are derived from a single gene product through differential processing.
    • The PG1 isozyme is sufficient to mediate both polyuronide solubilization and depolymerization in vivo during tomato fruit ripening.
    • This study elucidates the enzymatic basis of cell wall breakdown during ripening and the role of specific isozymes.