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Related Experiment Videos

Ethylene Biosynthesis-Inducing Xylanase : III. Product Characterization.

J F Dean1, K C Gross, J D Anderson

  • 1Plant Hormone Laboratory, Beltsville Agricultural Research Center, Beltsville, Maryland 20705.

Plant Physiology
|June 1, 1991
PubMed
Summary

Ethylene biosynthesis-inducing xylanase (EIX) from Trichoderma viride induces ethylene production in tobacco. Its activity depends on enzyme concentration and incubation time, with specific kinetic properties observed.

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Area of Science:

  • Plant biochemistry
  • Enzymology
  • Plant-microbe interactions

Background:

  • Ethylene biosynthesis is a crucial plant response pathway.
  • Xylanases are enzymes that degrade xylan, a major plant cell wall component.
  • Ethylene biosynthesis-inducing xylanase (EIX) is known to trigger ethylene production in plants.

Purpose of the Study:

  • To characterize the ethylene biosynthesis-inducing xylanase (EIX) from Trichoderma viride.
  • To investigate the enzymatic properties and substrate specificity of EIX.
  • To determine the relationship between EIX concentration, incubation time, and ethylene induction in tobacco.

Main Methods:

  • Isolation and purification of EIX from Cellulysin and Trichoderma viride cultures.
  • Enzyme kinetic studies (K(m), V(max)) at various temperatures to determine Arrhenius activation energies.

Related Experiment Videos

  • Analysis of EIX digestion products from purified xylans, ground corn cobs, and tobacco cell walls.
  • Assay of ethylene production in tobacco leaf discs upon incubation with EIX.

    • Main Results:

    • EIX-induced ethylene biosynthesis in tobacco leaf discs was dependent on xylanase concentration and incubation duration.
    • Arrhenius activation energies for Cellulysin and T. viride EIX were determined to be 9,100 and 10,500 calories, respectively.
    • EIX exhibited strictly endo-fashion xylan digestion, releasing polysaccharides from corn cobs but minimal carbohydrate from tobacco cell walls, with no debranching activity observed.

    Conclusions:

    • EIX from T. viride effectively induces ethylene biosynthesis in tobacco.
    • The enzyme's activity is concentration- and time-dependent, with specific kinetic parameters.
    • EIX primarily acts on xylan backbone, with limited impact on tobacco cell walls and no heat-stable ethylene-inducing products generated from purified xylans.