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Related Experiment Video

Updated: Jul 13, 2026

A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates
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Acid and Neutral Invertases in the Mesocarp of Developing Muskmelon (Cucumis melo L. cv Prince) Fruit.

A P Ranwala1, S S Iwanami, H Masuda

  • 1Department of Agricultural Chemistry, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080, Japan.

Plant Physiology
|July 1, 1991
PubMed
Summary

Activities of acid and neutral invertases in muskmelon fruit decrease during maturation, coinciding with sucrose accumulation. These enzymes, found in soluble and cell-wall fractions, show distinct properties and behaviors during purification.

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Area of Science:

  • Biochemistry
  • Plant Physiology
  • Enzymology

Background:

  • Invertases play a crucial role in carbohydrate metabolism in plants.
  • Understanding invertase activity is key to comprehending fruit ripening and sugar accumulation.

Purpose of the Study:

  • To characterize acid and neutral invertases in developing muskmelon fruit mesocarp.
  • To investigate the localization and properties of these enzymes during fruit maturation.

Main Methods:

  • Enzyme extraction from soluble and cell-wall fractions of muskmelon fruit mesocarp.
  • Enzyme activity assays and characterization (pH optima, substrate specificity, K(m), metal ion inhibition).
  • Chromatographic separation techniques (DEAE-cellulose, CM-cellulose) to differentiate enzyme isoforms.

Main Results:

  • Both acid and neutral invertases were detected, with activities declining as fruit matured and sucrose accumulated.
  • Acid invertase was found in soluble and cell-wall fractions, while neutral invertase was exclusively soluble.
  • Three types of cell-wall bound acid invertases were identified (NaCl-released, EDTA-released, tightly bound).
  • Soluble acid and neutral invertases differed in properties; cell-wall bound and soluble acid invertases showed similarities.

Conclusions:

  • Enzyme activities of acid and neutral invertases decrease during muskmelon fruit ripening.
  • Distinct properties and localization of invertase isoforms suggest specific roles in sucrose metabolism during fruit development.