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Sucrose Synthase Expression during Cold Acclimation in Wheat.

M D Crespi1, E J Zabaleta, H G Pontis

  • 1Centro de Investigaciones Biológicas, Fundación para Investigaciones Biológicas Aplicadas (F.I.B.A.), Casilla de Correo 1348, 7600 Mar del Plata, Argentina.

Plant Physiology
|July 1, 1991
PubMed
Summary
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Cold temperatures increase sucrose synthase (SS) in wheat seedlings. This study shows SS peptide and gene expression rise during cold acclimation, indicating its role in chilling stress response.

Area of Science:

  • Plant Physiology
  • Molecular Biology
  • Biochemistry

Background:

  • Plants acclimate to cold temperatures to survive. Cold stress impacts carbohydrate metabolism.
  • Sucrose synthase (SS) is a key enzyme in sucrose metabolism, but its role in cold acclimation is not fully understood.

Purpose of the Study:

  • To investigate the effect of cold temperature on sucrose synthase (SS) levels in wheat (Triticum aestivum) seedlings.
  • To determine if SS is involved in the plant's response to chilling stress.

Main Methods:

  • Wheat seedlings were exposed to cold (4°C) and warm (23°C) temperatures.
  • Western blot analysis was used to quantify SS peptide levels.
  • Northern blot analysis was performed to assess SS gene expression.

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Main Results:

  • Cold exposure (4°C) for 14 days increased SS peptide levels in wheat leaves.
  • Transferring plants back to warm temperatures (23°C) decreased SS levels.
  • Northern blot analysis revealed a five- to sixfold induction of SS gene expression during cold acclimation.

Conclusions:

  • Sucrose synthase (SS) is upregulated in wheat seedlings in response to cold stress.
  • Increased SS levels and gene expression suggest a significant role for SS in plant cold acclimation and chilling stress tolerance.