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Related Experiment Videos

Relationship between Electron Transport across the Plasmalemma and a pH Decrease in the Bulk Medium.

B Rubinstein1, A I Stern, J D Chalmers

  • 1Department of Botany, University of Massachusetts, Amherst, Massachusetts 01003.

Plant Physiology
|March 1, 1992
PubMed
Summary
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Proton excretion during transplasmalemma electron transport is not solely for charge balance. Even when negative charge remains constant, acidification occurs, challenging previous hypotheses.

Area of Science:

  • Plant Physiology
  • Biochemistry
  • Cell Biology

Background:

  • Transplasmalemma electron transport is crucial for cellular processes.
  • Acidification of the external medium is often observed during these processes.
  • The role of charge balance in this acidification is debated.

Purpose of the Study:

  • To investigate if acidification during transplasmalemma electron transport is solely due to charge balance requirements.
  • To elucidate the mechanism of proton excretion linked to ferricyanide reduction.

Main Methods:

  • Utilized oat (Avena sativa L. cv Garry) mesophyll protoplasts.
  • Employed a coulometric device for rapid reoxidation of ferrocyanide to ferricyanide.
  • Measured ferricyanide reduction and net proton excretion kinetics.

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Main Results:

  • Ferricyanide reduction and proton excretion kinetics in protoplasts were consistent with other cell types.
  • Net proton excretion rates were unaffected by simultaneous ferrocyanide reoxidation.
  • Acidification occurred even when the bulk medium's negative charge remained constant.

Conclusions:

  • Acidification during transplasmalemma electron transport can occur independently of net charge changes in the bulk medium.
  • The findings suggest mechanisms beyond simple charge balance contribute to proton excretion.
  • This challenges the hypothesis that acidification is solely a charge-balancing phenomenon.