Related Experiment Video
Updated: Aug 7, 2026

Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 14, 2016
Group 3 LEA Gene HVA1 Regulation by Cold Acclimation and Deacclimation in Two Barley Cultivars with Varying Freeze
F Sutton1, X Ding, D G Kenefick
1Plant Science Department, South Dakota State University, Brookings, South Dakota 57007.
Abstract:
The level of expression of the group 3 late embryogenesis abundant abscisic acid-regulated gene (HVA1) to cold treatment has been studied in winter barley (Hordeum vulgare) seedling tissue. The cDNA clone (pHVA1) encoding this late embryogenesis abundant protein was used as a hybridization probe to detect the corresponding mRNA. Expression of the HVA1 gene was determined after the tissue had been subjected to a regimen of 2 degrees C exposure (cold acclimation), followed by a return to 25 degrees C growth conditions (deacclimation). Accumulation of HVA1 mRNA occurred upon cold acclimation of the tissue and disappeared as early as 2 hours after exposure to deacclimation conditions. A comparison of the response to cold acclimation and deacclimation was made between seedling tissue of a freeze-resistant and less freeze-resistant cultivar. In both cultivars, the HVA1 gene was expressed and modulated by cold treatment. Within 2 hours of deacclimation HVA1 mRNA was no longer detectable in either cultivar independently of freeze resistance. The level of expression of HVA1 appeared to be greater in the less freeze-resistant cultivar (Winter Malt).
Related Concept Videos
Adaptations that Reduce Water Loss
Responses to Heat and Cold Stress
Cell Signaling in Plants

