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Nucleotide Sequence of a Complementary DNA Clone Encoding Stearoyl-Acyl Carrier Protein Desaturase from Simmondsia

A Sato1, C K Becker, V C Knauf

  • 1Nippon Steel Corporation Life Science Research Center, 1618 Ida, Nakahara-ku, Kawasaki 211 Japan.

Plant Physiology
|May 1, 1992
PubMed
Abstract

No abstract available in PubMed .

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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