Related Experiment Video
Updated: Aug 8, 2026
13C6-Glucose Labeling Associated with LC-MS: Identification of Plant Primary Organs in Secondary Metabolite Synthesis
Published on: March 22, 2024
Quantitative Analysis of Photosynthate Unloading in Developing Seeds of Phaseolus vulgaris L. : I. The Use of
E C Ellis1, R Turgeon, R M Spanswick
1Section of Plant Biology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853-5908.
Abstract:
The pathway and kinetics of photosynthate unloading in developing seeds of bean (Phaseolus vulgaris L.) were investigated using steady-state labeling with (14)CO(2). The continuous assimilation of (14)CO(2) at constant specific activity produced stable tracer fluxes that facilitated straightforward analyses of photosynthate import and unloading in developing seeds. The kinetics of tracer equilibration within intact seeds were compatible with a symplastic route of photosynthate unloading in the seed coat. The import and partitioning of tracer within seeds were partially disrupted by the surgical excision of the distal halves of seeds as practiced during the preparation of "empty" seed coats for perfusion.
