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Two Oryza sativa Genomic DNA Clones Encoding 16.9-Kilodalton Heat-Shock Proteins

S S Tzeng1, K W Yeh, Y M Chen

  • 1Department of Botany, National Taiwan University, Taipei, Taiwan, Republic of China.

Plant Physiology
|August 1, 1992
PubMed
Abstract

No abstract available in PubMed .

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Southern Blot02:57

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...