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Related Experiment Videos

Leptin affects oligodendroglial development in the mouse embryonic cerebral cortex.

Jun Udagawa1, Masayuki Nimura, Hiroki Otani

  • 1Research Project Promotion Institute, Shimane University, Izumo, Japan. jun@med.shimane-u.ac.jp

Neuro Endocrinology Letters
|May 4, 2006
PubMed
Summary

Leptin deficiency in mice leads to an increase in oligodendrocyte precursor cells (OPCs) in the embryonic brain. This suggests leptin normally inhibits OPC development, but does not directly act on these cells.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Endocrinology

Background:

  • Leptin, a product of the obese gene, regulates appetite and energy expenditure.
  • Previous studies indicate leptin influences neural stem and progenitor cells, including oligodendrocyte lineage cells.
  • Leptin deficiency is associated with altered glial protein levels, suggesting a role in glial cell development.

Purpose of the Study:

  • To investigate the role of leptin in oligodendrocyte development within the embryonic cerebral cortex.
  • To compare oligodendrocyte precursor cell populations in leptin-deficient (ob/ob) mice and wild-type controls.

Main Methods:

  • Utilized immunohistochemistry on embryonic day 18 (E18) mouse embryos (ob/ob and wild-type).
  • Employed antibodies against NG2, platelet-derived growth factor receptor-alpha (PDGFR-alpha), and leptin receptor (Ob-R).

Related Experiment Videos

  • Quantified and compared oligodendrocyte precursor cells (OPCs) positive for NG2 and/or PDGFR-alpha in the cerebral cortex.

    • Main Results:

    • Leptin-deficient (ob/ob) embryos exhibited a significantly higher number of OPCs compared to wild-type embryos at E18.
    • Oligodendrocyte precursor cells expressing PDGFR-alpha in the cerebral cortex did not co-express the leptin receptor.

    Conclusions:

    • Leptin appears to inhibit the differentiation of progenitor cells into OPCs in the developing mouse brain.
    • The findings suggest leptin's effect on OPCs is indirect, as OPCs themselves do not express the leptin receptor.