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Updated: Aug 8, 2026

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
Published on: May 13, 2020
Parity violating energetic difference and enantiomorphous crystalsp-caveats; reinvestigation of tyrosine
Meir Lahav1, Isabelle Weissbuch, Edna Shavit
1Department of Materials and Interfaces, Weizmann Institute of Science, 76100 Rehovot, Israel. meir.lahav@weizmann.ac.il
Abstract:
The present article challenges reports claiming to have demonstrated the Parity Violating Energetic Difference (PVED) between enantiomorphous D- and L-crystals. Apart from PVED, the presence of minute quantities and differing profiles of impurities incorporated during their different history of preparation will affect the physical properties of D- and L-crystals. These impurities are anticipated to play a much greater role in affecting crystallization behavior than PVED. The effect of impurities on the growth and dissolution of enantiomorphous crystals is illustrated with some representative examples. Shinitzky et al. (2002) reported recently dramatic differences in the growth and dissolution properties of the D- and L-crystals of tyrosine. We have repeated these experiments using commercial samples from different sources and employing a validated enantioselective gas chromatographic technique. We attribute Shinitzky's findings either to the use of inappropriate analytical techniques for the determination of enantiomeric composition and/or to the presence of unidentified contaminants in the commercial tyrosine samples. Related caveats hold also for the recently published claims by Shinitzky (2006) and Scolnik et al. (2006) to have observed experimentally PVED between enantiomeric helices of poly-glutamic acid composed of 24 repeating units.
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