Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Substrate-dependent contribution of double-stranded RNA-binding motifs to ADAR2 function.

Ming Xu1, K Sam Wells, Ronald B Emeson

  • 1Department of Pharmacology, School of Medicine, Vanderbilt University, Nashville, TN 37232-8548, USA.

Molecular Biology of the Cell
|May 5, 2006
PubMed
Summary

The two RNA-binding motifs in ADAR2 have distinct roles in RNA editing and cellular localization. These double-stranded RNA-binding motifs (dsRBMs) show substrate-specific functions, impacting adenosine to inosine conversion.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Insulin exits skeletal muscle capillaries by fluid-phase transport.

The Journal of clinical investigation·2018
Same author

Hypertension is associated with preamyloid oligomers in human atrium: a missing link in atrial pathophysiology?

Journal of the American Heart Association·2014
Same author

Reactive γ-ketoaldehydes promote protein misfolding and preamyloid oligomer formation in rapidly-activated atrial cells.

Journal of molecular and cellular cardiology·2014
Same author

[Changes of non-structural carbohydrates and its impact factors in trees: a review].

Ying yong sheng tai xue bao = The journal of applied ecology·2014
Same author

Sex-specific prevalence of fatty liver disease and associated metabolic factors in Wuhan, south central China.

European journal of gastroenterology & hepatology·2014
Same author

Insecticidal quinoline and isoquinoline isoxazolines.

Bioorganic & medicinal chemistry letters·2014

Area of Science:

  • Molecular Biology
  • Biochemistry
  • RNA Biology

Background:

  • ADAR2 (adenosine deaminase acting on RNA 2) is crucial for RNA editing via adenosine to inosine (A-to-I) conversion.
  • ADAR2 possesses two tandem double-stranded RNA-binding motifs (dsRBMs) essential for RNA substrate editing and nucleolar localization.
  • The functional distinction between these similar dsRBMs in ADAR2 remains unclear.

Purpose of the Study:

  • To investigate the specific roles of each dsRBM in ADAR2-mediated RNA editing and subnuclear localization.
  • To determine if dsRBMs recognize distinct features of RNA substrates.
  • To elucidate the differential contributions of dsRBMs to ADAR2's pleiotropic functions.

Main Methods:

  • Site-directed mutagenesis of ADAR2 dsRBMs.

Related Experiment Videos

  • Assays for RNA editing activity (A-to-I conversion).
  • Analysis of subnuclear localization using microscopy.

    • Main Results:

    • dsRBM1 modulates editing activity in a substrate-dependent manner.
    • dsRBM2 is essential for editing, but specific mutations affect only a subset of RNAs.
    • dsRBMs are interchangeable for nucleolar targeting but not for site-selective editing.
    • Each dsRBM exhibits unique binding preferences contributing to ADAR2 function.

    Conclusions:

    • The two dsRBMs of ADAR2 possess distinct functional specificities for RNA editing and localization.
    • Substrate-specific interactions mediated by dsRBMs are critical for ADAR2's pleiotropic roles.
    • Understanding dsRBM function provides insight into the regulation of RNA editing.