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Uranium(VI) reduction by Anaeromyxobacter dehalogenans strain 2CP-C
Qingzhong Wu1, Robert A Sanford, Frank E Löffler
1School of Civil and Environmental Engineering, Georgia Institute of Technology, 311 Ferst Drive, 3228 ES&T Building, Atlanta, GA 30332-0512, USA.
Applied and Environmental Microbiology
|May 5, 2006
Summary
Anaeromyxobacter dehalogenans strain 2CP-C reduces uranium(VI) to uranium(IV) using hydrogen as an electron donor. This bacterium shows metabolic versatility, making it a promising model for studying uranium reduction in contaminated environments.
Area of Science:
- Microbiology
- Environmental Science
- Geochemistry
Background:
- Anaeromyxobacter dehalogenans strain 2CP-C previously showed growth with various electron donors and acceptors.
- Uranium(VI) reduction is a critical process for uranium immobilization in contaminated environments.
Purpose of the Study:
- To investigate and characterize the reduction of Uranium(VI) by Anaeromyxobacter dehalogenans strain 2CP-C.
- To determine the optimal conditions and potential interferences for Uranium(VI) reduction by this bacterium.
Main Methods:
- Cell suspensions of fumarate-grown 2CP-C were used to test Uranium(VI) reduction.
- Growth studies were conducted using hydrogen or acetate as electron donors.
- The impact of various electron acceptors (nitrate, Fe(III) citrate, amorphous Fe(III) oxide, fumarate, 2-chlorophenol) on Uranium(VI) reduction was assessed.
Main Results:
- Hydrogen was identified as the essential electron donor for Uranium(VI) reduction, not acetate.
- Nitrate addition caused temporary Uranium(VI) reoxidation, but reduction resumed.
- Fe(III) citrate inhibited Uranium(VI) reduction, while amorphous Fe(III) oxide slowed but did not prevent it.
- Fumarate and 2-chlorophenol did not inhibit Uranium(VI) reduction and were consumed concurrently.
Conclusions:
- Anaeromyxobacter dehalogenans strain 2CP-C effectively reduces Uranium(VI) to Uranium(IV) with hydrogen as the electron donor.
- The bacterium's ability to utilize multiple electron acceptors and tolerate common contaminants suggests its potential role in uranium bioremediation.
- This strain serves as a valuable model for understanding complex microbial interactions in uranium-impacted sites.