Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mechanisms of amphotericin B-induced decrease in glomerular filtration rate in rats.

R Sabra1, R A Branch

  • 1Center for Clinical Pharmacology, University of Pittsburgh School of Medicine, Pennsylvania 15261.

Antimicrobial Agents and Chemotherapy
|December 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

River Plume Liftoff Dynamics and Surface Expressions.

Water resources research·2020
Same author

Pre-treatment apparent diffusion coefficient mapping: differentiation of benign from malignant laryngeal lesions.

The Journal of laryngology and otology·2015
Same author

Involvement of renal cytochromes P450 and arachidonic acid metabolites in diabetic nephropathy.

Journal of biological regulators and homeostatic agents·2013
Same author

A comparison of the effect of frusemide and bumetanide on the diuretic response and fibrinolytic mechanism in man.

British journal of clinical pharmacology·2012
Same author

Environmental procarcinogen hypothesis of bladder cancer in humans: Dapsone hydroxylation as a susceptibility risk factor for aggressive bladder cancer.

Urologic oncology·2011
Same author

Using team-based learning to teach clinical pharmacology in medical school: student satisfaction and improved performance.

Journal of clinical pharmacology·2010
Same journal

Isavuconazole for invasive mold disease in patients with hematological malignancies: a multicenter real-world study from China on efficacy, safety, and competing risks.

Antimicrobial agents and chemotherapy·2026
Same journal

An Achilles' heel for methicillin-resistant <i>Staphylococcus aureus</i>? Re-evaluating β-lactam susceptibility of MRSA.

Antimicrobial agents and chemotherapy·2026
Same journal

Essential role of glycogen synthase kinase 3 in regulating growth, drug response, and infectivity in <i>Leishmania infantum</i>.

Antimicrobial agents and chemotherapy·2026
Same journal

<i>In vitro</i> antibacterial activity of gepotidacin in combination with other antimicrobial agents against <i>Neisseria gonorrhoeae</i> isolates.

Antimicrobial agents and chemotherapy·2026
Same journal

Development of domain-specific probes of <i>Plasmodium falciparum</i> heat shock protein 70-1.

Antimicrobial agents and chemotherapy·2026
Same journal

Addressing therapeutic options for KPC-3-producing ST307-<i>Klebsiella pneumoniae</i>: insights from <i>in vitro</i> evolution and mutant prevention strategies.

Antimicrobial agents and chemotherapy·2026
See all related articles

This study investigated how amphotericin B affects kidney function in rats. Salt loading and theophylline protect against amphotericin B-induced reductions in renal blood flow (RBF) and glomerular filtration rate (GFR), suggesting calcium influx plays a key role.

Area of Science:

  • Nephrology
  • Pharmacology
  • Physiology

Background:

  • Amphotericin B is an antifungal agent known to cause nephrotoxicity.
  • Reductions in renal blood flow (RBF) and glomerular filtration rate (GFR) are key indicators of this toxicity.

Purpose of the Study:

  • To investigate the mechanisms underlying amphotericin B-induced reductions in RBF and GFR in rats.
  • To examine the influence of salt status and the roles of adenosine, cyclic AMP, and calcium influx.

Main Methods:

  • Seven groups of rats received intra-arterial infusions of amphotericin B.
  • RBF and GFR were measured before, during, and after infusion under varying salt conditions and with administration of theophylline, dibutyryl cyclic AMP, adenosine antagonist, or diltiazem.

Related Experiment Videos

Main Results:

  • Amphotericin B caused a 50% decrease in RBF and creatinine clearance (CLCR).
  • Salt loading, theophylline, and diltiazem administration prevented these decreases.
  • Adenosine did not appear to mediate these effects.

Conclusions:

  • Salt loading and theophylline demonstrate a protective effect against acute amphotericin B nephrotoxicity in rats.
  • Theophylline's protective mechanism is independent of adenosine receptor blockade or phosphodiesterase inhibition.
  • Calcium influx is likely responsible for the acute changes in RBF and GFR induced by amphotericin B.