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Bladder Smooth Muscle Strip Contractility as a Method to Evaluate Lower Urinary Tract Pharmacology
Published on: August 18, 2014
Effect of partial bladder outlet obstruction on nitrotyrosine levels and their correlation with contractile function
Anita Mannikarottu1, Alpha Dian-Yu Lin, Catherine Whitebeck
1Albany College of Pharmacy, Albany, New York 12208, USA. mannikaa@acp.edu
Neurourology and Urodynamics
|May 5, 2006
Summary
Partial bladder outlet obstruction (PBOO) increases nitrotyrosine, a marker of free radical damage, and impairs detrusor smooth muscle contractility. This suggests nitrotyrosine can indicate injury in obstructive bladder disease.
Area of Science:
- Urology
- Cellular Biology
- Pathophysiology
Background:
- Partial bladder outlet obstruction (PBOO) induces oxidative stress and cellular damage.
- Free radicals generated during PBOO contribute to tissue injury.
Purpose of the Study:
- To investigate the association between protein nitration and contractile dysfunction in obstructive bladder disease.
- To determine if nitrotyrosine levels correlate with functional changes in the bladder.
Main Methods:
- Rabbits underwent PBOO for 1-28 days, with sham-operated controls.
- Western blotting quantified nitrotyrosine levels.
- Contractile responses of isolated bladder strips were measured.
Main Results:
- Nitrotyrosine levels significantly increased by day 7 and remained elevated.
- Contractile dysfunction was evident early (day 1) and worsened progressively.
- Dysfunction in response to carbachol and KCl was significant after 3 days.
Conclusions:
- PBOO elevates nitrotyrosine, indicating free radical damage.
- Detrusor smooth muscle contractility progressively declines with obstruction.
- Nitrotyrosine may serve as a biomarker for free radical damage and reperfusion injury.
