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Updated: Aug 8, 2026

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts
Published on: March 8, 2017
Mapping electrochemiluminescence as generated at double-band microelectrodes by confocal microscopy under steady
Christian Amatore1, Cécile Pebay, Laurent Servant
1Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 PASTEUR, 24 rue Lhomond, 75231 Paris Cedex 05, France. christian.amatore@ens.fr
Abstract:
A confocal microspectrometer was used to collect spatially resolved electrochemiluminescence (ECL) spectra from the annihilation reaction between Ru(bpy)3+ and Ru(bpy)3(3+), each species being generated at a platinum band microelectrode and separated by a micrometric insulating gap. The use of a confocal microspectrometer allows in situ photon detection of the ECL reaction with micrometric spatial resolution and observes the concentration distribution of the photon source in the vicinity of the interelectrode gap.
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