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Isolation of Gln- mutants through transposon, Tn917, mutagenesis in Bacillus brevis
H S Mishra1, D P Mishra, G K Garg
1Department of Biochemistry, G.B. Pant University of Agriculture & Technology, Pantnagar.
Indian Journal of Biochemistry & Biophysics
|October 1, 1991
Abstract:
Transposon, Tn917, carried on pTV1 plasmid has been used successfully to mutagenise Bacillus brevis. The transposon showed preference for insertion at an "aro" site. A second insertional event after elimination of the preferred site with ethidium bromide/acridine orange treatment has permitted isolation of Gln- mutants in B. brevis.