Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Retinoid-binding proteins in craniofacial development.

L Dencker1, A L Gustafson, E Annerwall

  • 1Department of Toxicology, Uppsala University, Academic Hospital, Sweden.

Journal of Craniofacial Genetics and Developmental Biology
|October 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MR-guided adaptive versus ITV-based stereotactic body radiotherapy for hepatic metastases (MAESTRO): a randomized controlled phase II trial.

Radiation oncology (London, England)·2022
Same author

Network on veterinary medicines initiated by the European Federation For Pharmaceutical Sciences.

Journal of veterinary pharmacology and therapeutics·2017
Same author

Two-step kinetic model of the self-assembly mechanism for diphenylalanine micro/nanotube formation.

Physical chemistry chemical physics : PCCP·2017
Same author

Increased corneal densitometry as a subclinical corneal change associated with multiple myeloma.

Eye (London, England)·2017
Same author

[5-Year-Follow-up of the MEmbeR Multicenter Study on Medical-Occupational Rehabilitation].

Die Rehabilitation·2017
Same author

[Establishment of the new cross-sectional field of pain medicine : An application example at the medical faculty of Heidelberg].

Schmerz (Berlin, Germany)·2017
Same journal

Bone mineral density in the growing human mandible.

Journal of craniofacial genetics and developmental biology·2001
Same journal

Palate vault morphology in Down syndrome.

Journal of craniofacial genetics and developmental biology·2001
Same journal

Interrelation between fusions in the primary dentition and agenesis in the succedaneous permanent dentition seen from an embryological point of view.

Journal of craniofacial genetics and developmental biology·2001
Same journal

Correlation of HSP110 expression with all-trans retinoic acid-induced apoptosis.

Journal of craniofacial genetics and developmental biology·2001
Same journal

Secalonic acid D alters the expression and phosphorylation of the transcription factors and their binding to cAMP response element in developing murine secondary palate.

Journal of craniofacial genetics and developmental biology·2001
Same journal

Dentition development and budding morphogenesis.

Journal of craniofacial genetics and developmental biology·2001
See all related articles

Retinoic acid (RA) accumulates in developing neural crest cells and central nervous system (CNS) tissues. Cellular retinoic acid-binding protein (CRABP) localizes to these areas, suggesting RA

Area of Science:

  • Developmental Biology
  • Neuroscience
  • Genetics

Background:

  • Neural crest cells are crucial for craniofacial and CNS development.
  • Retinoids, including retinoic acid (RA), are known teratogens causing developmental malformations.
  • The precise role and localization of RA during early embryogenesis remain incompletely understood.

Purpose of the Study:

  • To investigate the distribution and localization of retinoic acid (RA) and its binding proteins within the developing mouse embryo.
  • To correlate RA localization with neural crest cell development and central nervous system (CNS) patterning.
  • To explore the potential role of RA as a morphogen in craniofacial and hindbrain development.

Main Methods:

  • Immunohistochemistry was used to localize cellular retinoic acid-binding protein (CRABP) and cellular retinol-binding protein (CRBP).

Related Experiment Videos

  • Saturable accumulation of RA congeners in specific embryonic tissues was assessed.
  • Localization patterns were analyzed in relation to neural crest cell migration pathways and CNS structures.

    • Main Results:

    • RA congeners and CRABP showed specific, saturable accumulation in neural crest cells, frontonasal mesenchyme, visceral arches, hindbrain (rhombomeres 4-6), and spinal cord.
    • CRABP co-localized with RA in these developing embryonic structures.
    • Retinol and CRBP distribution was more widespread, with specific expression in surface ectoderm, gut epithelium, and myocardium.

    Conclusions:

    • RA and CRABP are precisely localized in key developmental regions, including neural crest-derived tissues and the CNS.
    • These findings support a significant role for RA in the pattern formation of the craniofacial region and hindbrain.
    • RA may function as a morphogen during early embryonic development, similar to its proposed role in limb development.