Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective

Simon Ekström1, Lars Wallman, Daniel Hök

  • 1Department of Electrical Measurements, Lund Institute of Technology, Lund University, Lund, Sweden.

Journal of Proteome Research
|May 6, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-throughput acoustic FFPE proteomics reveals ATP5IF1-associated mitochondrial alterations in acral melanoma.

Molecular & cellular proteomics : MCP·2026
Same author

From Primary Melanoma to Metastatic Evolution: AI-Powered Pathology Integrated with Functional Analysis and Clinical Metadata Improving Treatment Prediction.

Cancers·2026
Same author

Phosphoproteomic profiling reveals post-translational dysregulation in Huntington's disease patient-derived neurons.

Cellular & molecular biology letters·2026
Same author

The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A.

Nature communications·2026
Same author

Molecular signatures of oocyte viability identified by follicular fluid proteomics.

Scientific reports·2026
Same author

A Proteomic Study of the Dual Oncogenic and Tumor-Suppressive Roles of SIRT3 in Lung and Breast Cancer Cell Lines.

International journal of molecular sciences·2026

A novel Integrated Selective Enrichment Target (ISET) device simplifies proteomic sample preparation for mass spectrometry. This microfabricated device enhances peptide detection and signal intensity compared to existing methods.

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is a key technique in proteomics.
  • Efficient sample preparation is crucial for maximizing peptide detection and data quality in MALDI MS.
  • Existing methods for peptide purification and concentration can be labor-intensive and vary in efficiency.

Purpose of the Study:

  • To introduce and validate a novel microfabricated device, the Integrated Selective Enrichment Target (ISET), for proteomic sample preparation.
  • To evaluate the performance of the ISET device for peptide purification and concentration.
  • To compare the ISET device's effectiveness against established sample preparation techniques.

Main Methods:

  • Development of a microfabricated device featuring an array of 96 perforated nanovials.

Related Experiment Videos

  • Loading nanovials with solid-phase extraction media for peptide purification and concentration.
  • Analysis of low nM-pM standard peptide samples and biological samples using the ISET device.
  • Comparative analysis of ISET with ZipTip and MassPREP PROtarget sample preparation methods using MALDI MS.

    • Main Results:

    • Demonstrated the validity of the ISET device for sample preparation using standard and biological samples.
    • ISET showed superior performance compared to ZipTip and MassPREP PROtarget.
    • ISET resulted in a higher number of detected peptides and increased signal intensity.

    Conclusions:

    • The Integrated Selective Enrichment Target (ISET) is a highly effective device for proteomic sample preparation.
    • ISET offers significant advantages over conventional methods in terms of peptide detection and signal enhancement.
    • This technology has the potential to advance proteomic analyses by improving data quality and throughput.