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Related Experiment Videos

Engineering three-dimensional pulmonary tissue constructs.

Mark J Mondrinos1, Sirma Koutzaki, Eugean Jiwanmall

  • 1School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania, USA.

Tissue Engineering
|May 6, 2006
PubMed
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Researchers engineered 3-D pulmonary tissue constructs using fetal pulmonary cells (FPC). Matrigel hydrogels supported alveolar development and gene expression, offering a potential path for lung tissue regeneration.

Area of Science:

  • Regenerative Medicine
  • Tissue Engineering
  • Pulmonary Biology

Background:

  • Engineering functional pulmonary tissue in vitro remains a significant challenge.
  • Fetal pulmonary cells (FPC) contain diverse cell types crucial for lung development.
  • Understanding 3-D culture requirements for distal lung epithelial cells is vital.

Purpose of the Study:

  • To engineer 3-D pulmonary tissue constructs using murine embryonic day 18 FPC.
  • To evaluate the efficacy of different biomaterials (Matrigel, PLGA, PLLA) in supporting lung tissue development.
  • To investigate the role of 3-D culture in inducing specific lung cell phenotypes and gene expression.

Main Methods:

  • Isolation and culture of murine embryonic day 18 FPC.
  • Fabrication of 3-D scaffolds using Matrigel, poly-lactic-co-glycolic acid (PLGA) foams, and poly-L-lactic-acid (PLLA) nanofibers.

Related Experiment Videos

  • Characterization of cell phenotype using immunohistochemistry, RT-PCR, and electron microscopy.
  • Assessment of tissue development and gene expression (SpC, FGFr2) in response to growth factors.

    • Main Results:

    • Matrigel hydrogels supported the formation of alveolar forming units (AFU) with alveolar type II (AE2) cell characteristics and SpC expression.
    • Tissue-specific growth factors induced branching epithelial structures and FGFr2 expression, essential for distal lung morphogenesis.
    • PLGA and PLLA scaffolds allowed FPC ingrowth but did not support distal lung epithelial cell survival.
    • 3-D culture was necessary to induce the expression of the morphogenesis-associated gene FGFr2.

    Conclusions:

    • Matrigel hydrogels provide a suitable microenvironment for engineering 3-D pulmonary tissue with key distal lung characteristics.
    • Synthetic scaffolds require further optimization to support distal lung epithelial cell survival and development.
    • These findings represent a foundational step towards engineering lung tissue for therapeutic applications in diseases like neonatal pulmonary hypoplasia.