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Array-comparative genomic hybridization to detect genomewide changes in microdissected primary and metastatic oral
Chung-Ji Liu1, Shu-Chun Lin, Yann-Jang Chen
1School of Dentistry, National Yang-Ming University, Taipei, Taiwan.
Molecular Carcinogenesis
|May 6, 2006
Summary
Genomic alterations in oral squamous cell carcinoma (OSCC) were mapped using array-CGH. Specific gene gains and losses were identified, with some differing between primary and metastatic tumors, aiding potential clinical evaluation.
Area of Science:
- Genomics
- Oncology
- Molecular Biology
Background:
- Oral squamous cell carcinoma (OSCC) is a prevalent global cancer.
- The genomic changes driving OSCC progression to invasive and metastatic stages remain largely unknown.
Purpose of the Study:
- To map genomewide genetic alterations in primary OSCC and metastatic tissues.
- To identify specific genomic changes associated with OSCC invasion and metastasis.
Main Methods:
- Array-based comparative genomic hybridization (array-CGH) was employed on microdissected primary and metastatic OSCC samples.
- Immunohistochemistry was used to assess protein expression of frequently altered genes in 25 primary and metastatic OSCC pairs.
Main Results:
- Frequent gains were observed in genes including LMYC, REL, TERC, PIK3CA, and MYB.
- Frequent losses were detected in genes such as p44S10, TIF1, LPL, MTAP, and BMI1.
- Significant differences in genomic alterations were found between primary and metastatic OSCC (e.g., TGFbeta2, CRBP1, PIK3CA) and between survivors and non-survivors (e.g., PRKCZ, ABL1, FGF4).
- High PIK3CA and FGF4 immunoreactivity correlated with worse prognosis, while CRBP1 loss was evident in both primary and metastatic OSCC.
Conclusions:
- Genomic profiling can identify specific alterations in OSCC progression.
- Identified genomic changes, such as PIK3CA and FGF4 alterations, may serve as potential biomarkers for prognosis and clinical evaluation in OSCC.
