Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dihydroxyacetone detoxification in Saccharomyces cerevisiae involves formaldehyde dissimilation.

Mikael Molin1, Anders Blomberg

  • 1Department of Cell and Molecular Biology, Microbiology, Göteborg University, Lundberg laboratory, Medicinaregatan 9c, S-413 90 Göteborg, Sweden.

Molecular Microbiology
|May 9, 2006
PubMed
Summary

This study reveals that yeast growing on dihydroxyacetone (DHA) activates formaldehyde detoxification pathways and glutathione metabolism. DHA toxicity is complex, involving advanced glycation end-products and stress responses beyond typical oxidative stress defenses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bronchial mucosal nuclear transcription factor expression and inflammatory response in humans after exposure to wood smoke.

Particle and fibre toxicology·2026
Same author

Sleep apnoea in patients undergoing colorectal cancer surgery: prospective cohort study.

BJS open·2026
Same author

Economic evaluation of long-term oxygen therapy: 24 hours versus 15 hours per day in severe hypoxemia-the REDOX trial.

Annals of the American Thoracic Society·2026
Same author

No association between troponin and COPD without cardiovascular influence: findings from a population-based cohort (SCAPIS).

Thorax·2026
Same author

Characterisation of chronic obstructive pulmonary disease (COPD) in never-smokers and ever-smokers from a population-based cohort.

BMJ open respiratory research·2026
Same author

Corrigendum to "Plasma proteins associated with cardiovascular disease in relation to lung function in SCAPIS" [Respir. Med. 249 (2025) 108463-108463].

Respiratory medicine·2026

Area of Science:

  • Microbiology
  • Biochemistry
  • Yeast Physiology

Background:

  • Dihydroxyacetone (DHA) is a conditionally toxic triose sugar.
  • Saccharomyces cerevisiae possesses two dihydroxyacetone kinase (DAK) isogenes, DAK1 and DAK2.
  • Understanding DHA metabolism is crucial for yeast physiology and biotechnology.

Purpose of the Study:

  • To investigate Saccharomyces cerevisiae physiology during growth on dihydroxyacetone (DHA).
  • To analyze protein expression in yeast strains overexpressing DAK1 or DAK2.
  • To elucidate mechanisms of DHA toxicity and tolerance.

Main Methods:

  • Comparative proteomic analysis of yeast strains grown on DHA.
  • Gene deletion and overexpression studies (SFA1, GSH1).
  • Assessment of formaldehyde dehydrogenase activity and glutathione synthesis.

Related Experiment Videos

Main Results:

  • DHA metabolism shares similarities with ethanol utilization but involves specific regulatory changes.
  • Formaldehyde detoxification pathway (Fdhlp) and glutathione metabolism (GSH1) are critical for DHA tolerance.
  • High DHA concentrations induce advanced glycation end-product (AGE) formation and stress-related proteins, but not canonical oxidative stress responses.

Conclusions:

  • Yeast detoxification of DHA involves complex interactions between formaldehyde metabolism and glutathione pathways.
  • DHA toxicity mechanisms are largely independent of central oxidative stress defense systems.
  • Evolutionary adaptation to minimize DHA's detrimental effects involves intricate metabolic regulation.