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Changing pattern of human group A rotaviruses: emergence of G12 as an important pathogen among children in eastern

S Samajdar1, V Varghese, P Barman

  • 1Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C. I. T. Road Scheme XM, Beliaghata, Kolkata 700010, West Bengal, India.

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
|May 9, 2006
PubMed

Insights

Rotavirus G12 strains have emerged as a significant cause of childhood diarrhea in eastern India. This finding highlights the need to include G12 in future rotavirus vaccines.

Area of Science:

  • Virology
  • Pediatrics
  • Public Health

Background:

  • Rotavirus genotypes G1-G4 and G9 are common causes of childhood diarrhea globally.
  • Previous studies in Kolkata detected G1, G2, G4, and G12 rotavirus strains.

Purpose of the Study:

  • To determine the prevalence of G- and P-genotypes of rotaviruses in children with dehydrating diarrhea.
  • To investigate rotavirus strains in eastern India.

Main Methods:

  • Active surveillance in two hospitals in eastern India (January 2003-April 2005).
  • Rotavirus detection using RNA electrophoresis.
  • G- and P-genotyping via RT-PCR and multiplex PCR.
  • Sequencing and phylogenetic analysis of VP7 genes for G12 strains.

Main Results:

  • G1 (53.8%) was the predominant genotype, followed by G2 (22.5%) and G12 (17.1%).
  • G3 and G4 strains were not detected.
  • Mixed P-types were most common (35.2%), followed by P[4] (31.7%), P[8] (21.8%), and P[6] (9.8%).
  • Phylogenetic analysis showed G12 strains clustered into three lineages globally.
  • G12 strains from Kolkata and Berhampur had conserved VP7 sequences but different P-types.

Conclusions:

  • Rotavirus G12 strains have emerged as an important pathogen in children in eastern India.
  • The emergence of G12 necessitates its inclusion in future polyvalent rotavirus vaccines.
  • Monitoring rotavirus genotypes is crucial for vaccine development and public health strategies.
Abstract

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