Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein adsorption onto polyester surfaces: is there a need for surface activation?

Björn Atthoff1, Jöns Hilborn

  • 1Polymer Chemistry, Department of Materials Chemistry, Uppsala University, Box 538, 75121 Uppsala, Sweden.

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
|May 9, 2006
PubMed
Summary

Surface hydrolysis of polyester scaffolds enhances protein adsorption, improving cell attachment. This treatment is crucial for non-degradable polyesters and affects protein layer morphology.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Time-dependent solvothermal synthesis of melamine cyanurate and melamine diborate: experimental and theoretical insights.

Physical chemistry chemical physics : PCCP·2025
Same author

Therapeutic Reprogramming of Glioblastoma Phenotypic States Using Multifunctional Heparin Nanoparticles.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Unveiling extracellular matrix assembly: Insights and approaches through bioorthogonal chemistry.

Materials today. Bio·2023
Same author

Technology platform for facile handling of 3D hydrogel cell culture scaffolds.

Scientific reports·2023
Same author

An Injectable, Shape-Retaining Collagen Hydrogel Cross-linked Using Thiol-Maleimide Click Chemistry for Sealing Corneal Perforations.

ACS applied materials & interfaces·2023
Same author

Biomimetic polyelectrolyte coating of stem cells suppresses thrombotic activation and enhances its survival and function.

Biomaterials advances·2023

Area of Science:

  • Biomaterials science
  • Surface chemistry
  • Polymer science

Background:

  • Surface hydrolysis is a proposed method to enhance protein adsorption on polyester scaffolds.
  • Improved protein adsorption is critical for promoting cell attachment in biomedical applications.

Purpose of the Study:

  • To investigate the impact of surface hydrolysis on protein adsorption across different polyesters.
  • To elucidate the conditions required for optimized protein adsorption and subsequent cell attachment.

Main Methods:

  • X-ray photoelectron spectroscopy (XPS) for surface composition analysis.
  • Scanning electron microscopy (SEM) for morphological characterization of protein coatings.
  • Quartz crystal microbalance (QCM) for quantifying protein adsorption.

Related Experiment Videos

Main Results:

  • Hydrolyzed poly(ethylene terephthalate) (PET) showed significantly higher protein adsorption than non-hydrolyzed.
  • Degradable polyesters (poly(lactic acid) - PLA, poly(glycolic acid) - PGA) exhibited increased adsorption rates but not amounts after hydrolysis.
  • XPS confirmed increased surface nitrogen on hydrolyzed PET, indicating thicker protein layers; SEM revealed distinct fibrous structures on hydrolyzed surfaces.

Conclusions:

  • Surface hydrolysis increases protein adsorption rates on polyesters, particularly inducing fibrous structures on non-degradable types.
  • While hydrolysis boosts adsorption on degradable polyesters, the total amount adsorbed remains similar.
  • Enhanced protein adsorption via hydrolysis and post-treatment significantly improves cell attachment to polyester surfaces.