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Related Experiment Videos

[Effect of self-microemulsifying system on cell tight junctions].

Xian-Yi Sha1, Xiao-Ling Fang

  • 1College of Pharmacy, Fudan University, Shanghai 200032, China. xianyi_sha@sina.com

Yao Xue Xue Bao = Acta Pharmaceutica Sinica
|May 11, 2006
PubMed
Summary

Positively charged self-microemulsifying systems (SMES) transiently disrupt intestinal cell tight junctions, enhancing mannitol permeability. The mechanism involves altered distribution of tight junction proteins ZO-1 and actin.

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Area of Science:

  • Cell biology
  • Nanotechnology
  • Pharmacology

Background:

  • Cellular tight junctions regulate paracellular transport.
  • Self-microemulsifying systems (SMES) are drug delivery vehicles.
  • Investigating charge-dependent effects of SMES on cellular barriers is crucial.

Purpose of the Study:

  • To investigate the impact of charged SMES on the intestinal epithelial tight junction complex.
  • To elucidate the molecular mechanisms underlying SMES-induced barrier modulation.

Main Methods:

  • Established a human intestinal epithelial Caco-2 cell model.
  • Assessed transepithelial electrical resistance (TEER) and mannitol permeability.
  • Evaluated tight junction protein (ZO-1) and actin localization via immunofluorescence.

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Main Results:

  • Negatively charged SMES had minimal impact on TEER.
  • Positively charged SMES significantly reduced TEER, with partial recovery observed.
  • Both SMES formulations enhanced mannitol permeability in a dose-dependent manner.
  • Immunofluorescence revealed altered ZO-1 and actin distribution, particularly with positively charged SMES.

Conclusions:

  • SMES can enhance paracellular transport.
  • Altered distribution of ZO-1 and actin is a potential mechanism for SMES-mediated tight junction opening.