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Published on: October 6, 2022
[Influence of LY294002 on sperm motility in asthenozoospermia patients in vitro]
Jun Yang1, Ji-hong Liu, Xiao-xin Gong
1Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. laseryang2003@163.com
Objective:
To investigate the effects of LY294002, a phosphatidylinositol 3-kinase inhibition, on sperm motility in asthenozoospermia patients in vitro, and further analyze the possible molecular mechanism.
Methods:
Sperm aseptically obtained by masturbation and prepared by swim-up technique from 10 patients with asthenozoospermia and 10 healthy fertile men were incubated with different concentrations of LY294002. Measurements of motility were carried out at 10, 30 and 60 min in all specimens by CASA.
Results:
The sperm in asthenozoospermia patients treated with LY294002 showed a significant increase in sperm progressive motility, the percentage of motile cells, VSL and VAP.
Conclusion:
LY294002 can enhance the motility of sperm in asthenozoospermia patients in vitro.
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