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Postprandial interstitial insulin concentrations in type 2 diabetes relatives
M Sandqvist1, E H Johanson, B Ahrén
1Department of Internal Medicine, Sahlgrenska Academy at Göteborg University, Sweden. madelene.sandqvist@medic.gu.se
European Journal of Clinical Investigation
|May 11, 2006
Summary
Interstitial insulin concentration in human adipose tissue was measured after a mixed meal. Insulin delivery to tissues is rapid and appears to be a nonsaturable process in individuals at high risk for type 2 diabetes.
Area of Science:
- Metabolic Research
- Endocrinology
- Human Physiology
Background:
- Endothelial barriers may impede insulin transport, contributing to insulin resistance.
- Interstitial insulin concentration (I-insulin) and kinetics in human adipose tissue post-meal were previously uncharacterized.
Purpose of the Study:
- To characterize interstitial insulin concentration and kinetics in human adipose tissue after a mixed meal.
- To investigate the effect of nateglinide on insulin delivery to adipose tissue.
Main Methods:
- Microdialysis was used to measure interstitial insulin concentration in eight nondiabetic first-degree relatives of type 2 diabetes patients.
- Measurements were taken after a mixed meal, with and without nateglinide administration.
- Adipose tissue blood flow and lipolysis were measured concurrently.
Main Results:
- Interstitial insulin concentration increased post-meal, with a more pronounced response when nateglinide was administered.
- The postprandial interstitial insulin to plasma insulin ratio remained similar between conditions.
- No significant differences were observed in the onset or responsiveness of insulin action in situ.
Conclusions:
- Microdialysis is a viable method for measuring interstitial insulin in human adipose tissue post-meal.
- Transendothelial insulin delivery is rapid and likely a nonsaturable process in this population.
- Findings support the understanding of insulin kinetics in individuals at risk for type 2 diabetes.
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