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Updated: Aug 8, 2026
Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
Published on: October 7, 2018
[Generation of transgenic mouse of dentin sialoprotein and transgene expression analysis]
Han-tang Sun1, Ming-zhen Xiao, Bu-ling Wu
1Department of Oral Medicine, College of Stomatology, Fourth Military Medical University, Xi'an 710032, Shanxi Province, China. hantang@fmmu.edu.cn
Purpose:
To generate the transgenic mouse model of DSP and perform transgene expression analysis by RT-PCR.
Methods:
Plasmid pcDNA3.1-CX was constructed by substituting promoter cbeta-actin for CMV promoter of pcDNA3.1, and the ultimate transgenic vector, pcDNA3.1-CX-dsp, was constructed by cloning DSP coding sequence into pcDNA3.1-CX. The pcDNA3.1-CX-dsp plasmid was linearized and microinjected into the male pronucleus of the zygotes. The tail DNA of pups was tested by PCR and Southern blot. A member of F1 generation of one positive mouse was used to perform transgene expression analysis by RT-PCR.
Results:
717 embryos were implanted to 29 recipient pseudopregnant mice, 4 of the 67 pups carrying the transgene. Expression of DSP was detected in a member of F1 generation of one positive mouse by RT-PCR.
Conclusion:
Founders of the DSP transgenic mouse were obtained successfully, and the expression of DSP was primarily confirmed.
