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Comparison of anti-RNA properties of normal and ellipticine functionalized alpha and beta-oligonucleotides
M Lavignon1, J R Bertrand, H Porumb
1UA 147 CNRS, U 140 INSERM, Institut G. Roussy, Villejuif, France.
Nucleic Acids Symposium Series
|January 1, 1991
Abstract:
RNA is not cleaved as a consequence of the binding of RNase H to the duplex between RNA and a complementary alpha-oligodeoxyribonucleotide (oligo). In consequence targets have been selected which do not a priori require the action of RNase H to inhibit genetic expression. Two models have been used: The Friend Murine Leukemia Virus (F-MuLV) and the synthesis of rabbit beta globin.alpha-oligos trigger specific inhibitions in both systems. The functionalisation in 5' with the intercalating agent 9-NH2-ellipticine renders the oligos resistant to degradation and allows a direct action on cells.