Related Experiment Video

Updated: Aug 8, 2026

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
10:26

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

Stop and go: anti-proliferative and mitogenic functions of the transcription factor C/EBPbeta

Thomas Sebastian1, Peter F Johnson

  • 1Laboratory of Protein Dynamics and Signaling, NCI-Frederick, Frederick, Maryland 21702-1201, USA.

Cell Cycle (Georgetown, Tex.)
|May 12, 2006
PubMed

Insights

Oncogene-induced senescence (OIS) involves cell cycle arrest and tumor suppression. The transcription factor C/EBPbeta is crucial for RasV12-induced senescence, acting as a tumor suppressor in mouse cells, but may promote cancer in others.

Area of Science:

  • Cell Biology
  • Molecular Oncology
  • Cancer Research

Background:

  • Oncogene-induced senescence (OIS) is a cell cycle arrest mechanism activated by oncogene expression.
  • OIS functions as a tumor suppression pathway, involving key regulators like Arf-p53 and RB.
  • The transcription factor C/EBPbeta is a downstream target of Ras signaling.

Purpose of the Study:

  • To review the dual role of C/EBPbeta in cell cycle regulation.
  • To discuss C/EBPbeta's function in both senescence and oncogenic transformation.
  • To highlight C/EBPbeta as an essential component in RasV12-mediated senescence.

Main Methods:

  • Review of existing scientific literature on C/EBPbeta and senescence.
  • Analysis of C/EBPbeta's role in RasV12-induced senescence in mouse embryo fibroblasts (MEFs).
  • Investigation of C/EBPbeta's regulation of E2F target genes via RB:E2F complexes.

Main Results:

  • C/EBPbeta is essential for RasV12-mediated senescence in MEFs.
  • C/EBPbeta induces cell cycle arrest by negatively regulating E2F target genes.
  • Evidence suggests C/EBPbeta exhibits tumor suppressor-like activity in MEFs.

Conclusions:

  • C/EBPbeta plays a critical role in initiating oncogene-induced senescence.
  • C/EBPbeta's function is context-dependent, showing both tumor suppressive and oncogenic roles.
  • Understanding C/EBPbeta's mechanisms is key to deciphering its role in cancer development.

More Related Videos

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness06:21

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Published on: May 7, 2018

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...