Hyperspectral imaging: a new approach to the diagnosis of hemorrhagic shock

Skin color changes and mottling are frequently described signs of hemorrhagic shock (HEM). Based on this, we developed a noninvasive, noncontact hyperspectral imaging system (HSI), which quantifies and depicts the surface tissue saturation of oxygen (SHSIO2) for each pixel in a region of interest (ROI). Our purpose was to assess HSI in a porcine HEM model. We hypothesized that HEM would cause decreases in SHSIO2 of the skin.

The HyperMed HSI system employs a spectral separator to vary the wavelength of light admitted to a digital camera. During image acquisition, a "hypercube" of images, each at a separate wavelength, is generated (at 5-nm intervals, from 500 to 600 nm). Then, the visible light spectrum for each pixel in the hypercube is compared by linear regression to standard spectra for oxyhemoglobin (OxyHb) and deoxyhemoglobin (DeoxyHb). The resulting fit coefficients for OxyHb and DeoxyHb are used to calculate SHSIO2 values for each pixel in the ROI. The mean values for OxyHb, DeoxyHb, and SHSIO2 across the ROI are calculated. Grayscale SHSIO2 pictures of the ROI are also generated, in which the brightness of each pixel is proportional to its value. Seventeen pigs, 36.4 +/- 0.11 kg, underwent standard preparation, and were maintained on ketamine and isoflurane. Normothermia was maintained (37 degrees C to 39 degrees C). The hemorrhage group (HEM, n = 9) underwent three blood withdrawals, each 10 mL/kg, with 15 minutes between withdrawals. After the third withdrawal, animals were resuscitated with lactated Ringer's and then shed blood. The control group (CTRL, n = 8) received intravenous fluids at 100 mL/h. HSI images were obtained of the inner hindlimb throughout.

All HEM animals showed linear decreases in both mean SHSIO2 and OxyHb values with blood loss, which were reversed by resuscitation. These changes were evident on the grayscale SHSIO2 pictures, but not to the naked eye, and paralleled those of invasively obtained arterial base excess and mixed venous oxygen saturation.

HSI is a promising noninvasive and noncontact tool for quantifying changes in skin oxygenation during HEM and resuscitation.