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Related Experiment Videos

Integron presence in a multiresistant Morganella morganii isolate.

Laura Rojas1, Teresa Vinuesa, Fe Tubau

  • 1Section of Microbiology, Department of Pathology and Experimental Therapeutics, Campus de Bellvitge, University of Barcelona, Barcelona, Spain.

International Journal of Antimicrobial Agents
|May 13, 2006
PubMed
Summary

A multiresistant Morganella morganii strain

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • A multiresistant strain of Morganella morganii was isolated from a patient with severe pathologies.
  • The isolate exhibited resistance to a wide range of antimicrobials, including beta-lactams, aminoglycosides, and tetracyclines.

Purpose of the Study:

  • To investigate the mechanisms underlying the multidrug resistance in the isolated Morganella morganii strain.
  • To identify genetic elements, such as plasmids and integrons, responsible for conferring antimicrobial resistance.

Main Methods:

  • Analysis of porins using sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and black lipid bilayer experiments.
  • Plasmid isolation and characterization, including detection and sequencing of integrons and gene cassettes.

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  • Direct sequencing of identified integrons to determine the genes responsible for resistance.

    • Main Results:

    • Porin analysis indicated that porins were not the primary cause of multidrug resistance in this strain.
    • A 6.6 kb plasmid, designated pML2003, was identified and found to contain two integrons.
    • One integron harbored cassettes conferring resistance to aminoglycosides (aadB) and chloramphenicol (catB3).
    • The second integron contained cassettes conferring resistance to beta-lactams (blaP1b) and streptomycin (aadA2).

    Conclusions:

    • The multidrug resistance in this Morganella morganii isolate is primarily mediated by a plasmid carrying two integrons.
    • These integrons contain specific gene cassettes that confer resistance to clinically significant classes of antibiotics.
    • Understanding these genetic mechanisms is crucial for managing infections caused by multiresistant bacteria.