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Reference values of immunoglobulin E in umbilical cord sera
Insights
This study established normal umbilical cord serum immunoglobulin E (IgE) levels in Cuban neonates. These findings provide crucial reference values for allergy prevention programs in Cuba.
Area of Science:
- Pediatrics
- Immunology
- Allergy Prevention
Background:
- The National Program for the Prevention of Allergic Diseases initiated IgE testing in umbilical cord serum in 1986.
- Previous research lacked specific normal IgE values for Cuban children, relying on international data.
- Establishing local reference values is essential for accurate diagnosis and management.
Purpose of the Study:
- To determine normal umbilical cord serum immunoglobulin E (IgE) concentrations in Cuban neonates.
- To establish a baseline for the National Program for the Prevention of Allergic Diseases in Cuba.
- To address the lack of country-specific reference values.
Main Methods:
- A cohort of 900 neonates was studied.
- Exclusion criteria included maternal factors known to elevate serum IgE.
- The microELISA-IgE technique was employed for precise measurement.
Main Results:
- The mean umbilical cord serum IgE level was determined to be 0.64 IU/ml.
- The lower limit for normal IgE was found to be 0.06 IU/ml.
- The upper limit for normal IgE was established at 6.83 IU/ml.
Conclusions:
- This research provides the first normal reference values for umbilical cord serum IgE in Cuban neonates.
- These established values are vital for the effective implementation of allergy prevention strategies in Cuba.
- The findings support the ongoing efforts in pediatric allergy research and public health initiatives.
Abstract:
The National Program for the Prevention of Allergic Diseases, including the determination of IgE concentrations in umbilical cord serum at birth began in 1986. Research was conducted to determine normal umbilical cord serum values in Cuban children since no previous research had been performed. The values consulted corresponded to children from other countries with different characteristics from ours. A total of 900 neonates were examined, excluding those whose mothers had been affected by factors that increase serum IgE concentrations. IgE values higher than 10 IU/ml were also regarded as abnormal. The microELISA-IgE technique, a heterogenous immunoenzymatic sandwich type assay, was used. As reference values, a 0.64 mean, 0.06 lower limit and a 6.83 upper limit were obtained.