Related Experiment Video

Updated: Aug 8, 2026

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
05:58

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

The scope of quantitative polymerase chain reaction assays in clinical molecular pathology

R D Malcomson1, C T McCullough, D J Bruce

  • 1Department of Pathology, University of Edinburgh Medical School, Teviot Place, Edinburgh EH8 9AG.

Clinical Molecular Pathology
|August 1, 1995
PubMed
Abstract

No abstract available in PubMed .

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Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
05:58

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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