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Related Experiment Videos

Amphotericin B induced abnormalities in human platelets.

K B Pastakia1, N E Brownson, D A Terle

  • 1Laboratory of Cellular Hematology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA.

Clinical Molecular Pathology
|October 1, 1996
PubMed
Summary
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Amphotericin B impairs platelet function and increases platelet adhesion to white blood cells, potentially explaining poor platelet recovery during combined therapy. This study investigated the in vitro effects of amphotericin B on platelet function.

Area of Science:

  • Hematology
  • Pharmacology
  • Immunology

Background:

  • Platelet transfusions are crucial for managing thrombocytopenia.
  • Amphotericin B is an antifungal agent used in treating serious fungal infections.
  • Concurrent use of amphotericin B and platelet transfusions has been associated with poor platelet recovery.

Purpose of the Study:

  • To investigate the in vitro effects of amphotericin B on platelet function.
  • To elucidate the mechanisms behind poor platelet recovery in patients receiving both amphotericin B and platelet transfusions.

Main Methods:

  • Washed human platelets were exposed to amphotericin B (4 µg/ml) in vitro.
  • Platelet function assessed via aggregation, serotonin release, hypotonic stress response, and mean platelet volume.

Related Experiment Videos

  • Surface marker expression (GPIb-IX, GPIIb-IIIa, P-selectin) analyzed by flow cytometry.
  • Platelet-polymorphonuclear leukocyte (PMN) adhesion measured via flow cytometry.

    • Main Results:

    • Amphotericin B induced platelet dysfunction, inhibiting aggregation, serotonin uptake, and response to hypotonic stress.
    • Mean platelet volume increased up to two-fold.
    • P-selectin (CD62P) was expressed on unactivated platelets, and platelet adherence to PMNs increased.
    • Expression of GPIb-IX and GPIIb-IIIa remained unaffected.

    Conclusions:

    • Amphotericin B induces P-selectin expression on unactivated platelets and enhances platelet-PMN adhesion in vitro.
    • This platelet dysfunction and increased adhesion, potentially worsened by storage, may contribute to poor platelet recovery in patients receiving concomitant amphotericin B and platelet transfusions.