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Updated: Aug 8, 2026

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
Published on: March 29, 2017
Novel primer specific false terminations during DNA sequencing reactions: danger of inaccuracy of mutation analysis
R Anwar1, A Booth, A J Churchill
1Molecular Medicine Unit, University of Leeds, Department of Medicine, Clinical Sciences Building, St James's University Hospital, Leeds LS9 7TF.
Abstract:
The determination of nucleotide sequence is fundamental to the identification and molecular analysis of genes. Direct sequencing of PCR products is now becoming a commonplace procedure for haplotype analysis, and for defining mutations and polymorphism within genes, particularly for diagnostic purposes. A previously unrecognised phenomenon, primer related variability, observed in sequence data generated using Taq cycle sequencing and T7 Sequenase sequencing, is reported. This suggests that caution is necessary when interpreting DNA sequence data. This is particularly important in situations where treatment may be dependent on the accuracy of the molecular diagnosis.
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