Cryodamage on sperm chromatin according to different freezing methods, assessed by AO test
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1Infertility Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Siriraj Hospital, Bangkok, Thailand. somsin101@yahoo.com
Objective:
To evaluate cryodamage effects on human sperm chromatin, motility and cryosurvival rate after freeze-thawing, compared between liquid nitrogen vapour and computerized program freezer, assessed by acridine orange staining method (AO test).
Material And Method:
Fifty semen samples were used. After semen analysis, each semen sample was mixed with cryoprotective media and divided into 2 straws. The first straw was frozen with liquid nitrogen vapour and the second with computerized program freezer. After 1 month of cryostorage, semen samples were thawed. Sperm chromatin integrity, motility, morphology, vitality and sperm cryosurvival rate were determined.
Results:
DNA damage was significantly greater (p < 0.001) following freezing with liquid nitrogen vapour than with computerized program freezer. Furthermore, the computerized program freezing method significantly provided superior post-thaw sperm motility, vitality and cryosurvival rate, compared with the liquid nitrogen vapour freezing method.
Conclusion:
Computerized program freezing is recommended as a first choice method for routine cryostorage.
