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Related Experiment Video

Updated: Jun 19, 2026

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

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The bone marrow functionally contributes to liver fibrosis.

Francesco P Russo¹, Malcolm R Alison, Brian W Bigger

¹Department of Medicine, Imperial College, London, United Kingdom.

Gastroenterology

|May 16, 2006

Summary

This summary is machine-generated.

Bone marrow cells significantly contribute to liver fibrosis by forming myofibroblasts, not parenchymal regeneration. This finding is crucial for liver fibrosis therapies and clinical trials involving bone marrow cell transplantation.

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Cell Type-specific Gene Expression Profiling in the Mouse Liver

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Published on: September 17, 2019

Area of Science:

Hepatology
Stem Cell Biology
Fibrosis Research

Background:

Bone marrow (BM) cells can potentially transdifferentiate or fuse with organ cells.
Bone marrow (BM) cell therapy shows promise for treating cirrhosis in animal models.
However, BM cells may also contribute to scar-forming myofibroblasts in organs like the liver.

Purpose of the Study:

To investigate the role of bone marrow (BM) cells in liver regeneration and fibrosis.
To assess the temporal and functional contribution of BM-derived myofibroblasts in murine cirrhosis models.

Main Methods:

Induction of cirrhosis in female mice using carbon tetrachloride or thioacetamide.
Administration of male bone marrow (BM) transplants to lethally irradiated female mice.
Tracking of BM-derived cells using Y chromosome in situ hybridization and transgenic mouse strains to detect collagen production.

Main Results:

BM contributed minimally (0.6%) to parenchymal regeneration but significantly (68-70%) to hepatic stellate cell and myofibroblast populations in cirrhotic livers.
BM-derived myofibroblasts were transcriptionally active for collagen type 1 and influenced the fibrotic response.
These myofibroblasts originated from BM mesenchymal stem cells, not through cell fusion.

Conclusions:

Bone marrow (BM) cells play a significant functional role in liver fibrosis.
BM-derived myofibroblasts represent a potential therapeutic target for liver fibrosis.
Clinical trials using BM cell therapy for liver regeneration must monitor for potential enhancement of organ fibrosis.