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Morphine modulates monocyte-macrophage conversion phase.

Ikuske Hatsukari1, Naoko Hitosugi, Amit Dinda

  • 1Long Island Jewish Medical Center, New Hyde Park, NY 11040, USA.

Cellular Immunology
|May 16, 2006
PubMed
Summary
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Morphine inhibits monocyte-macrophage conversion phase (MMCP), potentially preventing monocyte migration. Opiate antagonists and superoxide dismutase (SOD) modulated these effects, suggesting complex roles in tissue injury.

Area of Science:

  • Immunology
  • Pharmacology

Background:

  • Monocyte migration and differentiation into macrophages are critical in tissue injury.
  • The monocyte-macrophage conversion phase (MMCP) is a key process in this response.

Purpose of the Study:

  • To investigate the effect of morphine on the MMCP.
  • To explore the role of opiate receptors and superoxide production in morphine's effect on monocytes.

Main Methods:

  • THP-1 cells were activated with Phorbol 12-myristate 13-acetate (PMA) to induce MMCP.
  • The influence of morphine, opiate receptor antagonists, and superoxide dismutase (SOD) on MMCP and superoxide production was assessed.
  • Monocyte adhesion and migration were measured.

Main Results:

Related Experiment Videos

  • Morphine inhibited PMA-induced MMCP, an effect reversed by opiate antagonists.
  • Both PMA and morphine stimulated superoxide production in monocytes.
  • SOD inhibited PMA-mediated MMCP and attenuated morphine's inhibitory effect.
  • PMA enhanced monocyte adhesion and migration, while morphine appeared to inhibit monocyte migration.

    • Conclusions:

    • Morphine inhibits the monocyte-macrophage conversion phase (MMCP).
    • Morphine may prevent monocyte migration by inhibiting MMCP.
    • These findings suggest potential therapeutic implications for morphine in modulating inflammatory responses and tissue injury.