Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional characterization of Src-interacting Na/K-ATPase using RNA interference assay.

Man Liang1, Ting Cai, Jiang Tian

  • 1Department of Physiology, Pharmacology, Metabolism, and Cardiovascular Sciences, Medical University of Ohio, Toledo, Ohio 43614, USA.

The Journal of Biological Chemistry
|May 16, 2006
PubMed
Summary

The sodium-potassium pump (Na/K-ATPase) forms a complex with Src kinase, regulating its activity and acting as a receptor for ouabain signaling. Altering Na/K-ATPase levels impacts Src activity and downstream signaling pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intelligent Localization of Cross-Sectional Structural Damage in Molten Salt Receiver Tubes Using Mel Spectrograms and TSA-Optimized 2D-CNN.

Sensors (Basel, Switzerland)·2026
Same author

Altered static and dynamic regional homogeneity in basal ganglia-thalamocortical circuits and their association with neuropsychiatric manifestations in Wilson's disease.

Frontiers in neuroscience·2026
Same author

Electrolyte-Replacement-Free Continuous Electrocatalytic Desalination Coupled With CO<sub>2</sub> Reduction at Record Throughput and Low Cost.

Angewandte Chemie (International ed. in English)·2026
Same author

Multi-Scale Genetic and Transcriptomic Analyses Identify Druggable Targets for Epilepsy.

Current medical science·2026
Same author

Development and validation of a new instrument to assess risk of falls among infants and toddlers.

BMC public health·2026
Same author

Integrated Metabolomic and Transcriptomic Analysis Reveals Mechanisms Underlying Increased Nitrogen and Protein Concentrations by Phosphorus Deficiency in Soybean Seeds.

Journal of agricultural and food chemistry·2026

Area of Science:

  • Cellular signaling
  • Molecular cell biology
  • Biochemistry

Background:

  • The sodium-potassium pump (Na/K-ATPase) is crucial for maintaining cellular ion balance.
  • Src kinase is a non-receptor tyrosine kinase involved in various cellular processes.
  • The interaction between Na/K-ATPase and Src suggests a role in signal transduction.

Purpose of the Study:

  • To investigate how Na/K-ATPase quantity and properties influence basal Src activity.
  • To determine the effect on ouabain-induced signal transduction pathways.
  • To elucidate the role of Na/K-ATPase as a signaling receptor complex.

Main Methods:

  • Generation of alpha1 subunit knockdown LLC-PK1 cell lines using small interference RNA.
  • Assessment of Na/K-ATPase activity and Src activity.

Related Experiment Videos

  • Analysis of tyrosine phosphorylation of focal adhesion kinase and ERK1/2 activation.
  • Rescue experiments with rat alpha1 subunit and mutant alpha1 (D371E).
  • Fluorescence resonance energy transfer (FRET) and co-immunoprecipitation assays.

    • Main Results:

    • Alpha1 knockdown decreased Na/K-ATPase activity but increased basal Src activity and focal adhesion kinase phosphorylation.
    • Ouabain-induced activation of Src and ERK1/2 was abolished in knockdown cells.
    • Rescue with rat alpha1 restored Na/K-ATPase and basal Src activity, with altered ouabain sensitivity.
    • A pumping-null alpha1 mutant (D371E) bound Src and restored basal Src activity and focal adhesion kinase phosphorylation.

    Conclusions:

    • A pool of Src-interacting Na/K-ATPase regulates basal Src activity in LLC-PK1 cells.
    • Na/K-ATPase functions as a receptor for ouabain, mediating protein kinase activation.
    • The Na/K-ATPase-Src complex is a key regulator of cellular signaling pathways.