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Hydrophobic binding sites on human interferon.

M W Davey, J W Huang, E Sulkowski

    The Journal of Biological Chemistry
    |January 10, 1975
    PubMed
    Summary
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    Human interferon binds selectively to hydrophobic chromatography resins, indicating a potential purification method. This interaction, driven by hydrophobic forces, allows for the separation of interferon from other proteins.

    Area of Science:

    • Biochemistry
    • Protein Chemistry
    • Chromatography

    Background:

    • Human interferon is a crucial therapeutic protein.
    • Purification of interferon is essential for its therapeutic applications.
    • Understanding interferon's binding properties can lead to improved purification strategies.

    Purpose of the Study:

    • To investigate the binding characteristics of human interferon to different agarose-based chromatography resins.
    • To determine the nature of the interaction between human interferon and hydrophobic ligands.
    • To assess the potential of hydrophobic chromatography for human interferon purification.

    Main Methods:

    • Affinity chromatography using omega-carboxypentyl-agarose and omega-aminohexyl-agarose.
    • Elution studies using varying ionic strengths (NaCl) and ethylene glycol.

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  • Analysis of protein binding and elution profiles.

    • Main Results:

    • Human interferon exhibited strong binding to omega-carboxypentyl-agarose, reversible by ethylene glycol, indicating hydrophobic interaction.
    • Binding to omega-aminohexyl-agarose was weak and likely electrostatic.
    • The omega-carboxypentyl-agarose column selectively retained human interferon, allowing other proteins to pass through.

    Conclusions:

    • Human interferon possesses a hydrophobic binding site, potentially adjacent to a positive charge.
    • Hydrophobic chromatography using omega-carboxypentyl-agarose is a selective and effective method for human interferon purification.
    • This approach offers a promising strategy for obtaining highly purified interferon for therapeutic use.