Related Experiment Video

Updated: Aug 8, 2026

An Efficient Method for Adenovirus Production
10:06

An Efficient Method for Adenovirus Production

Published on: June 10, 2021

Description of an adenovirus A31 outbreak in a paediatric haematology unit

M Leruez-Ville1, M Chardin-Ouachée, B Neven

  • 1Laboratoire de Virologie, EA 3620 Université Paris V, Hôpital Necker-Enfants Malades AP-HP, Paris, France. marianne.leruez@nck.ap-hop-paris.fr

Bone Marrow Transplantation
|May 16, 2006
PubMed

Insights

Adenovirus A31 caused an epidemic in pediatric stem cell transplant patients. This study highlights the need to assess the burden of this specific adenovirus genotype in this vulnerable population.

Area of Science:

  • Virology
  • Pediatric Hematology
  • Infectious Diseases

Background:

  • Allogeneic haematopoietic stem cell transplantation (hSCT) in children carries a high risk of adenovirus infections, leading to significant morbidity and mortality.
  • While species C and B adenoviruses are most common post-hSCT, adenovirus A31 has emerged as a growing concern.

Purpose of the Study:

  • To investigate an outbreak of adenovirus A31 infections in pediatric hSCT patients.
  • To characterize the clinical features and epidemiological spread of adenovirus A31 genotype.

Main Methods:

  • Quantitative real-time PCR was used to screen blood samples from 58 hSCT children for adenovirus species A-C over three years.
  • Phylogenetic analysis, including hexon gene amplification and sequencing, was performed on detected adenovirus strains.

Main Results:

  • Fifteen cases of adenovirus viraemia were identified in the study period.
  • Seven cases occurring in spring/summer 2003 were identified as adenovirus species A, specifically genotype A31, with 100% hexon gene homology.
  • Clinical data for these seven patients with A31 adenovirus viraemia were documented.

Conclusions:

  • An epidemic spread of adenovirus genotype A31 was observed in a pediatric hematology unit.
  • Epidemiological data strongly suggest a nosocomial (hospital-acquired) origin for this A31 adenovirus outbreak.
  • Further assessment of the burden of adenovirus A31 infection in hSCT recipients is warranted.

More Related Videos

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems13:47

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems

Published on: April 23, 2012

Related Concept Videos

Respiratory Syncytial Virus Disease01:29

Respiratory Syncytial Virus Disease

Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...
Poliomyelitis01:17

Poliomyelitis

Poliomyelitis is caused by poliovirus, a small, non-enveloped, positive-sense RNA virus of the Picornaviridae family and Enterovirus genus. Transmission occurs primarily via the fecal-oral route, often through ingestion of contaminated water or food. The virus initially replicates in the oropharynx and intestinal mucosa, particularly in lymphoid tissues such as the tonsils, Peyer’s patches, and regional lymph nodes. Primary viremia follows, allowing dissemination throughout the body.In most...
Influenza01:27

Influenza

Influenza is an acute, highly communicable viral disease that affects the respiratory tract and is responsible for seasonal epidemics worldwide. Influenza A is the most prevalent type associated with widespread outbreaks and is subtyped based on two surface glycoproteins: hemagglutinin (H) and neuraminidase (N), as in H1N1. These glycoproteins are essential for viral infectivity, transmission, and immune recognition. Transmission occurs primarily through respiratory droplets and contaminated...
Acute Pharyngitis01:30

Acute Pharyngitis

Introduction
Acute pharyngitis is the inflammation of the back of the throat (pharynx), commonly resulting in a sore throat. It is a frequently encountered condition that prompts individuals to seek medical advice.
Classification
Acute pharyngitis can be categorized based on its underlying cause:
Viral Meningitis01:18

Viral Meningitis

Viral meningitis is the most common form of meningitis and is often referred to as aseptic meningitis to indicate the absence of bacterial involvement. It is generally milder than bacterial meningitis, with symptoms including fever, headache, stiff neck, drowsiness, nausea, photophobia, and vomiting. Rarely, more severe manifestations or death may occur. Common causative agents include enteroviruses, particularly coxsackie A and B viruses and echoviruses, all members of the Enterovirus genus...