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Updated: Sep 29, 2026

Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
Published on: September 7, 2011
[Secretory IgA: quality control of the titration method]
F Rosaschino1, R Torre, R Grisler
1Istituto di Pediatria, Università di Milano.
Abstract:
In order to verify if the method for the titration of salivary secretory IgA (s-IgA) by the technical of radial immunodiffusion is trustworthy, have been carried out the precision tests in the series and among the series in two saliva samples with very different s-IgA concentrations, and precision and accuracy tests in the series and among the series using a solution with a known titre of IgA. The results of the first battery tests have shown a precision ranging from 93.04 to 95.43%. The results of the tests of the second battery have shown a precision ranging about 96% and an accuracy about 97%. The tested method is, therefore, sufficiently trustworthy.
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