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Related Experiment Videos

Oligo(A) not coded by DNA generating 3'-terminal heterogeneity in a lambda phage RNA.

G R Smith, J Hedgpeth

    The Journal of Biological Chemistry
    |June 25, 1975
    PubMed
    Summary

    Researchers discovered that a specific RNA molecule in E. coli, infected with phage lambda, has a variable length due to extra adenylate residues at its 3' end. These non-coded residues were removed, revealing a uniform RNA molecule.

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    Area of Science:

    • Molecular Biology
    • Bacteriology
    • Virology

    Background:

    • Escherichia coli (E. coli) is a common bacterium.
    • Phage lambda is a virus that infects E. coli.
    • RNA transcription involves synthesizing RNA from a DNA template.

    Purpose of the Study:

    • To investigate the structure and variability of a specific RNA species in E. coli infected with phage lambda.
    • To identify the cause of length heterogeneity in this RNA molecule.

    Main Methods:

    • Purification of RNA by hybridization to lambda 1-strand DNA.
    • Analysis of RNA length variability.
    • Treatment with Pancreatic RNase to assess 3'-terminal modifications.

    Main Results:

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    • The purified RNA species exhibited variable length.
    • Length variation was attributed to a variable number of 3'-terminal adenylate residues.
    • RNase treatment resulted in a homogeneous RNA molecule ending in -Up, indicating non-templated adenylation.

    Conclusions:

    • The studied RNA transcript possesses non-coded adenylate residues at its 3' terminus.
    • This suggests a post-transcriptional modification process occurring in E. coli during phage lambda infection.